IF4E Antibody [AMM22187G]

IF4E Antibody [AMM22187G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H, M, Rat

Specificity: This IF4E antibody is generated from a mouse immunized with a recombinant protein of human IF4E.

Cellular Localisation: Cytoplasm, P-body. Cytoplasm. Cytoplasm, Stress granule. Nucleus Note=Interaction with EIF4ENIF1/4E-T is required for localization to processing bodies (P-bodies) (PubMed:16157702, PubMed:24335285, PubMed:25923732) . Imported in the nucleus via interaction with EIF4ENIF1/4E-T via a piggy-back mechanism (PubMed:10856257)

Molecular Weight: 25097

Clone: Monoclonal

Gene Name: EIF4E (HGNC:3287)

Gene ID: 1977

Function: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures (PubMed:16271312, PubMed:22578813) . In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm (PubMed:24335285) . Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression: in the complex, EIF4E mediates the binding to the mRNA cap (By similarity) . Component of a multiprotein complex that sequesters and represses translation of proneurogenic factors during neurogenesis (By similarity) . In P-bodies, component of a complex that mediates the storage of translationally inactive mRNAs in the cytoplasm and prevents their degradation (PubMed:24335285) . May play an important role in spermatogenesis through translational regulation of stage-specific mRNAs during germ cell development (By similarity) .

Summary: N/A

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB, IF

Dilution: IF--1:25 WB--1:4000

Synonyms: Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F