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IFN-gamma (Human) ELISA Kit - E4825

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SKU:
26-E4825-100-GEN
Availability:
Usually shipped in 5 working days
NULL1,043.00

Description

E4825 | IFN-gamma (Human) ELISA Kit DataSheet

IFN-gamma is produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. ’s IFN-gamma (Human) ELISA kit is based on Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-gamma. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human IFN-gamma and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IFN-gamma bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IFN-gamma. The concentration of Human IFN-gamma in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : IFNG, IFG, IFI, Type II Interferon

Tag Line : A Sandwich ELISA kit for the quantitative measurement of human IFN-γ in plasma, tissue homogenates and other biological fluids.

Summary :

Detection Method : Sandwich ELISA, Absorbance (450 nm)

Sample Type : Plasma,Serum,Other biological fluids

Species Reactivity : Human

Applications : in vitro quantitative measurement of human IFN-γ in plasma, tissue homogenates and other biological fluids.

Features and Benefits : Specificity: No Significant cross-reactivity or interference between Human IFN-γ and analogues was observed.,Detection Range: 15.63-1000 pg/mL,Precision: Coefficient of variation is < 10%.,Sensitivity: 9.38 pg/mL

 

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Additional Information

Storage Conditions:
4ºC
Shipping Conditions:
Gel Pack
Shelf life:
12 months
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