IFT88 Antibody | 45-762

IFT88 Antibody | 45-762 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat, Dog

Immunogen: The immunogen for this antibody is: C-KKRIDEDDFADEE

Research Area: Cancer

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines HepG2 and NIH3T3 and in Human Kidney lysates (calculated MW of 85.3kDa according to Human NP_001340507.1 and 90.4kDa according to NP_001340498.1, and 93.1kDa according to Mouse NP_033402.2 93. and 89.2kDa according to XP_006518873.1) . Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration: 3ug/ml.Immunofluorescence: Expression of the protein seen within the nucleus and cytoplasm of HepG2 cells. Recommended concentration: 10ug/ml. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct;23 (10) :3289-97, PMID: 19470799.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognise many reported isoforms.

Positive Control 1: Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive) , Tg737 protein, intraflagellar transport 88 homolog, recessive polycystic kidney disease protein Tg737 homolog, intraflagellar transport 88 homolog (Chlamydomonas)

User Note: N/A

BACKGROUND: N/A