IGF-BP3, human recombinant | 4720

Human IGF-BP3, also known as growth-hormone-dependant binding protein, is a 28.9 kDa cysteine-rich secreted protein. It is the major IGF binding protein present in the plasma of human and animals. In addition to its ability to modulate the activity of IGF-I and IGF-2, IGF-BP3 also exerts inhibitory effects on follicle stimulating hormone (FSH) activity. Decreased plasma levels of IGF-BP3 often results in dwarfism, whereas elevated levels of IGF-BP3 may lead to acromegaly. The expression of IGF-BP3 in fibroblasts is stimulated by mitogenic growth factors such as Bombesin, Vasopressin, PDGF, and EGF. Recombinant human IGF-BP3 is a 28.8 kDa protein consisting of 264 amino acid residues.

4720 | IGF-BP3 human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Insulin-like Growth Factor-Binding Protein 3, Growth-hormone-dependant binding protein

Alternates names: Malate dehydrogenase, ECK3225, JW3205

Taglines: A protein which binds and prolongs the half-life of the IGFs

NCBI Gene ID #: N/A

NCBI Gene Symbol: N/A

Gene Source: Bacteria

Accession #: N/A

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 450 units/mg, and is defined as the amount of enzyme that cleaves 1 µmole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 34.9 kDa (336 aa, 1-312 aa + NT His Tag)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized from 20 mM HCl

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK