IGFBP-3 (Human) ELISA Kit - E4884

E4884 | IGFBP-3 (Human) ELISA Kit DataSheet

Insulin-like growth factor binding protein-3 (IGFBP-3) is a 264 amino acid peptide that belongs to the family of IGF-binding proteins. It is expressed in multiple tissues. One of the main functions of IGFBP-3 is that it regulates the transport, bioavailability, and half-life of insulin-like growth factors, especially IGF-1. IGFBP-3 is inversely correlated with cancer. Increased levels of IGFBP-3 in the serum inhibit proliferation and promote apoptosis of cancer cells, thus serving as a potential target for cancer research. ’s IGFBP-3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the amount of IGFBP-3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of IGFBP-3 in the sample or standard.

Alternate Name : IBP3, BP-53, IGFBP-3, BP-53, IBP-3, IGFBP3, Binding Protein 53, Binding Protein 29

Tag Line : A Sandwich ELISA kit for the quantitative measurement of IGFBP-3 in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma,Serum,Tissue lysate,Other biological fluids

Species Reactivity : Human

Applications : This Sandwich ELISA kit is designed to quantitatively measure amount of IGFBP-3 in human serum, plasma, tissue lysates and other biological fluids

Features and Benefits : Recovery range: between 85 - 105% for normal human serum and plasma samples,This Sandwich ELISA is highly sensitive and highly specific for the detection of IGFBP-3 in human samples. There is no significant cross-reactivity or interference between IGFBP-3 and analogues,Sensitivity: 0.234 ng/ml,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,Detection range: 0.391 - 25 ng/ml