IKKα/β (Phospho-S180/181) polyclonal Antibody | AP0625

IKKα/β (Phospho-S180/181) polyclonal Antibody | AP0625 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB

Application Range: WB: 1:2000~1:5000

Background: The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IKKα/β (Phospho-S180/181) polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at Ser180/181.

Molecular Weight: ~ 75 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; CHUK; IKKA, TCF16

Immunogen: Synthetic phosphopeptide derived from human IKKα/β around the phosphorylation site of Serine 180/181.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Phosphoinosotode Signaling,PI3K AKT signaling,Protein Kinase C Signaling,Immune Cell Signaling,Warburg Effect,Translational Contral elF4 and p70 S6 Kinase,Regulation of Microtubule Dynamics,T cell receptor signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,Cell Cycle Control G1 S Checkpoint,Adherens Junction signaling,Mitochondrial Control of Apoptosis,MTOR signaling,ErbB HER Signaling,