IKKγ (Phospho-S31) polyclonal Antibody | AP0266

IKKγ (Phospho-S31) polyclonal Antibody | AP0266 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IF IHC

Application Range: WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

Background: Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates I˚Bα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IKKγ (Phospho-S31) polyclonal Antibody detects endogenous levels of IKKγ protein only when phosphorylated at Ser31.

Molecular Weight: ~ 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier; IKBKG; FIP3, NEMO

Immunogen: Synthetic phosphopeptide derived from human IKKγ around the phosphorylation site of Serine 31.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Tumor Angiogenesis,NF-kB Signaling,Phospholipase Signaling,Signaling Pathways Activating p38 MAP Kinase,T cell receptor signaling,TGFβ Smad signaling,Toll-like Receptor signaling,Translational Contral elF4 and p70 S6 Kinase,