IL-1 beta, rat recombinant | 4130

IL-1 is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 and IL-1 binds to the same receptor and has similar biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, B-cell maturation and proliferation, mitogenic FGF-like activity and stimulation of the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 is a secreted cytokine, IL-1 is predominantly a cell-associated cytokine. Recombinant rat IL-1 is a 17.4 kDa protein containing 153 amino acid residues.

4130 | IL-1 beta rat recombinant DataSheet

Biomolecule/Target: IL-1 beta

Synonyms: Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF)

Alternates names: Protein phosphatase 1G, PP2CG, PPP2CG, MGC1675, MGC2870, PP2CGAMMA.

Taglines: An important mediator of inflammatory responses

NCBI Gene ID #: 5496

NCBI Gene Symbol: PPM1G

Gene Source: Human

Accession #: O15355

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 85%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Specific activity: >5,000 units/mg.

Results: >5,000 units/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 61.4 kDa (566 aa, 1-546 aa + NT His-Tag)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and then lyophilized from 10 mM Sodium Acetate, pH 6.5

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGelR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD