IL-11 Antibody | 8453

IL-11 Antibody | 8453 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%) , Mouse: (88%)

Immunogen: IL-11 antibody was raised against a 16 amino acid peptide from near the amino terminus of human IL-11.
The immunogen is located within amino acids 40 - 90 of IL-11.

Research Area: Stem Cell, Immunology

Tested Application: E, WB, ICC, IF

Application: IL-11 antibody can be used for the detection of IL-11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: IL-11 antibody is human specific. At least two isoforms of IL-11 are known to exist; this antibody will only detect the longest isoform of IL-11.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 20 kDa
Observed: 19 kDa

Validation: N/A

Isoform: N/A

Purification: IL-11 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-11 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Interleukin-11, Adipogenesis inhibitory factor, AGIF

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Interleukin 11 is a pleiotropic cytokine that has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6 (1) . It directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (2) . IL-11 promotes the proliferation of hepatocytes in response to liver damage. IL-11 binds to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 (3) . Clinically it is used to prevent severe thrombocytopenia and the reduction of the need for platelet transfusion following myelo-suppressive chemotherapy (4) .