IL-12, human recombinant | 4161

IL-12 is a potent regulator of cell mediated immune responses and in induces IFN- production by NK and T cells. It is produced by activated monocytes/macrophage cells, B-lymphocytes, and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+, and CD8+ cells and induce the development of IFN- producing Th1 cells. Recombinant human IL-12 is a 75 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (503 total amino acid residues).

4161 | IL-12 human recombinant DataSheet

Biomolecule/Target: IL-12

Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-, IL-12

Alternates names: Plasminogen activator inhibitor-1, SERPINE1, PAI, PAI1, PLANH1.

Taglines: A T cell-stimulating factor

NCBI Gene ID #: 5054

NCBI Gene Symbol: SERPINE1

Gene Source: Human

Accession #: P05121

Recombinant: Yes

Source: E. Coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: Specific activity, the IC50 value is < 56 nM. Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

Results: <56 nM

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 45 kDa (400 aa, 24-402 aa + NT His-Tag)

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized in 0.5X PBS, pH 7.2

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP