IL-2 Antibody [5B9-2-1] | 38-299

IL-2 Antibody [5B9-2-1] | 38-299 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: E.coli derived Recombinant Murine IL-2

Research Area: Infectious Disease, Neuroscience, Cancer, Stem Cell, Chemokines & Cytokines, Functional Assays

Tested Application: E, WB, Neut

Application: ELISA:
Sandwich:
In a sandwich ELISA (assuming 100μL/well) , a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with ProSci’s Polyclonal biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μg/mL.
Western Blot:
To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL) , a concentration of 10.0-14.0 μg/mL of this antibody is required.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: This IgG2Ak antibody was purified from cell culture by Protein A affinity chromatography.

Clonality: Monoclonal

Clone: 5B9-2-1

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine

User Note: Centrifuge vial prior to opening.

BACKGROUND: N/A