IL-2 (Rat) ELISA Kit - E4831

E4831 | IL-2 (Rat) ELISA Kit DataSheet

Interleukin-2 (Il-2) is produced by T-cells in response to antigenic or mitogenic stimulation. It is is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 can also stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. ’s IL-2 (Rat) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Rat IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of IL-2 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat IL-2. The concentration of Rat IL-2 in the samples can be calculated by comparing the OD of the samples to the standard curve.

Alternate Name : T-cell growth factor,IL2, TCGF, Lymphokine

Tag Line : A Sandwich ELISA kit for the quantitative measurement of rat IL-2 in serum, plasma and other biological fluids.

Summary :

Detection Method : Sandwich ELISA, Absorbance (450 nm)

Sample Type : Plasma,Serum,Other biological fluids

Species Reactivity : Rat

Applications : A Sandwich ELISA kit for the quantitative measurement of rat IL-2 in serum, plasma and other biological fluids.

Features and Benefits : Detection Range: 31.25-2000 pg/mL,Precision: Coefficient of variation is < 10%,Specificity: This kit recognizes Rat IL-2 in samples. No Significant cross-reactivity or interference between Rat IL-2 and analogues was observed.,Sensitivity: 18.75 pg/mL