IL-21 Antibody | 38-212

IL-21 Antibody | 38-212 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Mouse

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-21 (Murine Interleukin-21) . Murine IL-21 specific antibody was purified by affinity chromatography employing immobilized mIL-21 matrix.

Research Area: Immunology, Chemokines & Cytokines, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
Indirect:
To detect mIL-21 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.
Sandwich
To detect mIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Murine IL-21 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.
Western Blot:
To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: IL-21 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: Interleukin-21IL-21

User Note: Centrifuge vial prior to opening.

BACKGROUND: A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.