IL-27 Antibody | 3797

IL-27 Antibody | 3797 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (63%)

Immunogen: Anti-IL-27 antibody (3797) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human IL-27.
The immunogen is located within the first 50 amino acids of IL-27.

Research Area: Immunology, Cancer

Tested Application: E, WB, ICC

Application: WB: 0.5 - 4 μg/mL; ICC: 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 17-207 - EL4 Cell Slide

Positive Control 3: Cat. No. 1288 - A20 Cell Lysate

Positive Control 4: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 5: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 6: Cat. No. 1220 - SK-N-SH Cell Lysate

Molecular Weight: Predicted: 28 kDa
Observed: 28 kDa

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar IL-27 expression profile in human and mouse cell lines detected by two independent anti-IL-27 antibodies that recognize different epitopes, 3797 against N-terminus domain and 3799 against central domain.  IL-27 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 3) shows that anti-IL-27 antibody (3797) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 3797 antigenic peptide.

Isoform: Human IL-27 has one isoform (243aa, 28kD) . Mouse IL-27 has one isoform (234aa, 27kD) and rat IL-27 also has one isoform (234aa, 27kD) . 3797 can detect human, mouse and rat IL-27.

Purification: IL-27 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-27 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-27 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IL-27 Antibody: p28, IL30, IL-27, IL27A, IL-27A, IL27p28, Interleukin-27 subunit alpha, p28, IL-27 subunit alpha

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IL-27 Antibody: Like interleukin-23 (IL-23) , IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet.