IL-33 Antibody [IL33305B] | 36-355

IL-33 Antibody [IL33305B] | 36-355 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human IL-33.

Research Area: Chemokines & Cytokines

Tested Application: E, WB, Func, IHC, IP

Application: ELISA: (direct or indirect: 1:2'000-1:5'000) , Functional Application (Blocking) , Immunohistochemistry (paraffin sections (1:50-1:200) ) , Immunoprecipitation Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

Specificiy: Recognizes human IL-33. Does not cross-react with mouse IL-33.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G-affinity purified.

Clonality: Monoclonal

Clone: IL33305B

Isotype: IgG2aκ

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: Interleukin-33; IL-1F11; NF-HEV

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Interleukin-33 (IL-33; HF-NEV; IL-1F11) , a member of the IL-1 family of cytokines, is expressed by many cell types following pro-inflammatory stimulation and is thought to be released on cell lysis. The 30kDa human IL33 is converted by CASP1 to a 18kDa protein. IL33 binds to and signals through ST2 (IL1R1) and its stimulation recruits MYD88, IRAK, IRAK4, and TRAF6, followed by phosphorylation of ERK1 (MAPK3) /ERK2 (MAPK1) , p38 (MAPK14) , and JNK. The ability of IL-33 to target numerous immune cell types, like Th2-like cells, mast cells, and B1 cells, and to induce cytokine and chemokine production underlines its potential in influencing the outcome of a wide range of diseases, such as arthritis, asthma, atopic allergy & anaphylaxis, cardiovascular disease/atherosclerosis, nervous system diseases, and sepsis.