IL-34 Antibody | 4779

IL-34 Antibody | 4779 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: IL-34 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human IL-34.
The immunogen is located within amino acids 30 - 80 of IL-34.

Research Area: Signal Transduction

Tested Application: E, WB

Application: IL-34 antibody can be used for the detection of IL-34 by Western blot at 0.25 μg/mL. Despite its predicted molecular weight, IL-34 will often run at higher molecular weight in SDS-PAGE.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 26 kDa
Observed: 42-48 kDa

Validation: N/A

Isoform: N/A

Purification: IL-34 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IL-34 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IL-34 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IL-34 Antibody: IL-34, C16orf77, Interleukin-34, IL-34

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IL-34 Antibody: Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF) -1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.