223

IL-5 Antibody (biotin) | XP-5191Bt

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SKU:
223-XP-5191Bt-GEN
NULL614.00

Description

IL-5 Antibody (biotin) | XP-5191Bt | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human IL-5) .

Research Area: Immunology, Chemokines & Cytokines, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
Sandwich:
To detect hIL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-5 (XP-5191) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:
To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-hIL-5 specific antibody was purified by affinity chromatography and then biotinylated.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Biotin

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: IL-5 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: EDF, TRF, IL-5Interleukin-5, B-cell differentiation factor I, IL-5

User Note: Centrifuge vial prior to opening.

BACKGROUND: Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor) , functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70% amino acid sequence homology.

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Additional Information

Size:
0.05 mg
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