IL36B (Human) ELISA Kit | E5058-100

IL36B (Human) ELISA Kit | E5058-100

Description: Interleukin‑36 beta (IL36B) belongs to the IL‑1 family of proteins (1 ‑ 3, 6). IL36B binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Cells expressing IL-36 beta include resting and activated monocytes and B cells. The receptor for IL-36 beta is a blend of IL-1 Rrp2 and IL-1 RAcP. Recombinant IL-36 beta stimulates processes involving NF-kB and MAPK in an IL-1 Rrp2-dependent manner. In primary human joint cells (synovial fibroblasts and articular chondrocytes) IL-36β exerts proinflammatory effects, but serum levels of IL-36β do not correlate with joint inflammation. ’s IL36B (Human) ELISA Kit is used for the quantitative detection of IL36B in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-w. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of IL36B in the sample.

Alternate Name: IL36B ELISA Kit; Interleukin-36 beta ELISA Kit; FIL1 eta ELISA Kit; IL-1 eta ELISA Kit; IL1F8 ELISA Kit; IL-1H2 ELISA Kit; family of interleukin 1-eta ELISA Kit; FIL1 ELISA Kit; FIL1- ELISA Kit; ETA ELISA Kit; FIL1H ELISA Kit; IL1-ETA ELISA Kit; IL-1F8 ELISA Kit; IL1F8 ELISA Kit; Canonical product IL-1F8a ELISA Kit; IL-1F8 ELISA Kit; FIL1-eta ELISA Kit; IL1H2 ELISA Kit; interleukin 1 family ELISA Kit; member 8 ELISA Kit; eta ELISA Kit; interleukin 1 ELISA Kit; eta ELISA Kit; Interleukin 36 ELISA Kit; Beta ELISA Kit; Interleukin-1 eta ELISA Kit; interleukin-1 family member 8 ELISA Kit; Interleukin-1 homolog 2 ELISA Kit; Interleukin-1 Superfamily ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of IL36B

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of IL36B in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Detection range: 31.25-2000 pg/ml Highly sensitive and specific Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Recovery Rate: 87-101% for serum, 86-96% for EDTA plasma and 86-100% for heparin plasma Sensitivity: 18.75 pg/ml