IL4I1 Antibody (C-Term) [ApR04979G]

IL4I1 Antibody (C-Term) [ApR04979G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This IL4I1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 391-422 amino acids from human IL4I1.

Cellular Localisation: Secreted. Lysosome {ECO:0000250|UniProtKB:O09046}. Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Secreted at the immunological synapse.

Molecular Weight: 62881

Clone: Polyclonal

Gene Name: IL4I1 {ECO:0000303|PubMed:16029492}

Gene ID: 259307

Function: Secreted L-amino-acid oxidase that acts as a key immunoregulator (PubMed:17356132, PubMed:32818467, PubMed:32866000) . Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively (PubMed:17356132, PubMed:32818467, PubMed:32866000) . Also has weak L-arginine oxidase activity (PubMed:26673964) . Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR (PubMed:32818467, PubMed:32866000) . IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A) ) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer (PubMed:32818467, PubMed:32866000) . AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity (PubMed:32818467) . Also has an immunoregulatory function in some immune cells, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4 (+) T-cells into FOXP3 (+) regulatory T- cells (Treg) and regulates the development and function of B-cells (PubMed:17356132, PubMed:25446972, PubMed:25778793, PubMed:28891065) . Also regulates M2 macrophage polarization by inhibiting T-cell activation (By similarity) . Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4 (+) and H2O2 (PubMed:23355881) .

Summary: Tissue Location: Primarily found in immune tissues, with the highest expression in lymph nodes and spleen (PubMed:12031486, PubMed:12446450) . Present in germinal center macrophages and inflammatory myeloid cells and antigen-presenting cells (at protein level) (PubMed:17356132) . Also present in spermatozoa (at protein level) (PubMed:25767141) . Highly expressed in primary mediastinal large B-cell lymphoma, a specific subtype of diffuse large B-cell lymphoma (PubMed:12446450) . Expressed by neoplastic cells of several B-cell lymphomas and by tumor-associated macrophages (PubMed:19436310)

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: L-amino-acid oxidase, LAAO, LAO, 1.4.3.2, Interleukin-4-induced protein 1, IL4-induced protein 1, Protein Fig-1, hFIG1, IL4I1, FIG1