171
Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7 | T0352
- SKU:
- 171-T0352-GEN
- Availability:
- IN STOCK
Description
Immortalized Dog Mesenchymal Stem Cells-HPV E6/E7 | T0352
Immortalization Method: N/A BioSafety Level: II Organism: Canine Species: Dog Source Organ: Adipose Tissue Organ Type: Adipose Growth Properties: Adherent Morphology: Fibroblast-like Passage Number: N/A Population Doubling: 50-60 hours Seeding Density: N/A Markers: Puromycin Donor Age: N/A Donor Gender: N/A Donor Ethnicity: N/A Propagation: The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%, Penicillin/Streptomycin Solution to a final concentration of 1%, and 0.2 ?g/ml puromycin (G264). Carbon dioxide (CO2): 5%, Temperature: 37.0°C. Quality Control: 1. Real Time PCR was used to quantify HPV E6 and E7 gene expression in immortalized cell line. 2. Puromycin selection Shipping Condition: Dry Ice Storage Condition: liquid nitrogen or -180C
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.