171
Immortalized Feline Aortic Endothelial Cells - SV40 & hTERT | T0516
- SKU:
- 171-T0516-GEN
- Availability:
- IN STOCK
Description
Immortalized Feline Aortic Endothelial Cells - SV40 & hTERT | T0516
Immortalization Method:
Co-transduced with non-replicating lentivirus carrying SV40LT and hTERT
BioSafety Level:
II
Organism:
Feline
Species:
Feline
Source Organ:
Aorta
Organ Type:
Blood Vessel
Growth Properties:
Adherent
Morphology:
Elongated
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Markers:
N/A
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10%, 0.1 mg/ml gentamycin, 1 mM sodium pyruvate (TM057), 1% non-essential amino acids(TM068), 50 ?g/ml endothelial cell growth supplement (ECGS; Biomedical Technologies Inc.), and 10 U/ml heparin (Sigma; H5515) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Immunodetection for hTERT and SV40L T antigen
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C