Immortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP) | T0017

Immortalized Human Cord Blood Mesenchymal Stromal Cells with RFP (cbMSC-hTERT-RFP) | T0017

in vivostem cell research and translational study.

Immortalization Method:

Serial passaging and transfection with Human Telomerase Reverse Transcriptase (hTERT) expression vector pGRN145

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Umbilical cord blood

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

Population doubling time of 23.8 ± 1.9 h

Seeding Density:

Subculture ratio of 1:3 to 1:5

Markers:

CD90, CD34, CD44, CD45, CD166, CD117,CD73, CD105, CK18 and CK19

Donor Age:

N/A

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%, 4 ng/ml bFGF (Cat. Z101455), and Penicillin/streptomycin at a final concentration of 1%. It is recommended to renew medium every 2 to 3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) RT-PCR was used to confirm the expression of the hTERT transgene in the immortalized cells; 2) TRAP assay was performed to determine the telomerase activity; 3) Flow cytometry was used to detect surface markers such as CD29, CD73, CD105, CD44 and HLA-ABC; 4) G-banding was used for karyotype analysis; 5) Tumuorigenicity assay was performed to evaluate the cells’ tumorigenic potential.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C