Immortalized Human Cranial Periosteal Cells (Tag58) | T0716

Immortalized Human Cranial Periosteal Cells (Tag58) | T0716

Immortalization Method:

Immortalized with lentivirus containing large SV40 T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Bone

Organ Type:

Skeletal

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

CD44, CD73, CD90, CD105, CD146, CD166, MSCA-1 and low expression of CD34; neomycin resistance (0.25 mg/mL G418)

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

The osteogenic differentiation medium for this cell line is complete culture medium plus the following supplements: 10 mM ß-glycerophosphate disodium salt hydrate (Sigma), 100 μM L-ascorbic acid 2-phosphate (Sigma), and 4 μM dexamethasone (Sigma).
The osteogenic differentiation medium should be always prepared fresh.

Quality Control:

1) Osteogenic differentiation experiments; 2) Western blot; 3) Flow cytometry; 4) Detection of cell mineralization by fluorescent OsteoImage staining; 5) Gene expression analysis

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C