Immortalized Human Delta F508 Cystic Fibrosis Cells | T0738

Immortalized Human Delta F508 Cystic Fibrosis Cells | T0738

Immortalization Method:

Cocultured with irradiated 3T3 fibroblast feeder cells in the presence of the RhoA kinase inhibitor Y-27632.

BioSafety Level:

II

Organism:

Human (H. sapiens)

Species:

Human

Source Organ:

Airway/Lung

Organ Type:

Airway/Lung

Growth Properties:

Adherent

Morphology:

Epithelial

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

MUC5AC/MUC5B

Donor Age:

Not disclosed

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

These cells require inactivated 3T3J2 feeder cells to grow. Plate inactivated 3T3J2 cells on G422-coated culture vessels at a seeding density of 47,000 cells/cm². Allow cells to attach for 18 - 24 hours before adding Immortalized Human Delta F508 Cystic Fibrosis Cells.

The base medium for this cell line is a 1:3 mixture of Prigrow III medium and Prigrow XII medium available at abm.'s and To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 7%, Penicillin/Streptomycin Solution to a final concentration of 1%, L-glutamine to a final concentration of 2 mM, hydrocortisone to a final concentration of 25 ng/ml, Recombinant Human EGF to a final concentration of 0.125 ng/ml, Insulin to a final concentration of 10 µg/ml, cholera toxin to a final concentration of 8.6 ng/ml, and Y-27632 to a final concentration of 5 µM.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Whole mount immunofluorescence

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C