Immortalized Human Hair Follicle Dermal Papilla Cells –SV40T/c-Myc | T0503

Immortalized Human Hair Follicle Dermal Papilla Cells –SV40T/c-Myc | T0503

in vitro and to accelerate hair growth in mice models. A list of up-regulated genes in the DPC can be found in the supporting document section below. The DPC serves as a useful tool for the study of hair growth regulation.

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen and c-Myc gene

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Hair follicles

Organ Type:

Hair Follicle

Growth Properties:

Adherent

Morphology:

Flat|Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

20,000 - 30,000 cells/cm10², Recommended Split ratio of 1:2

Markers:

smooth muscle alpha actin, alkaline phosphatase

Donor Age:

Donor Info Not Disclosed

Donor Gender:

Donor Info Not Disclosed

Donor Ethnicity:

Donor Info Not Disclosed

Propagation:

The base medium for this cell line is Prigrow III medium available from abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and 1% Penicillin/streptomycin.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot was used to identify SV40T and c-myc transgene expression. 2) Immunocytochemistry and western blot were performed to assess the expression of smooth muscle actin alpha and alkaline phosphatase. 3) Up-regulated genes in DPCs is analyzed by DAVID (the Database for Annotation, Visualization and Integrated Discovery).

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C