Immortalized Human Hepatic Sinusoidal Endothelial Cells-SV40 | T0056

Immortalized Human Hepatic Sinusoidal Endothelial Cells-SV40 | T0056

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

12-22 hours

Seeding Density:

20, 000 cells/cm10²; Recommended split ratio is 1:3 to 1:4

Markers:

N/A

Donor Age:

Adult

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow I medium available from abm . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%, Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C