Immortalized Human Periodontal Ligament Fibroblasts - SV40 | T0044

Immortalized Human Periodontal Ligament Fibroblasts - SV40 | T0044

Immortalization Method:

Transduction with lentiviral SV40Tt (abm Cat. No. LV614)

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Periodontal Ligament

Organ Type:

Oral

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

30-40 hours

Seeding Density:

5,000 - 10,000 cells/cm10²

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is PriGrow II medium available at abm. to a final concentration of 10%, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C