Immortalized Human Synovial Membrane Fibroblasts -SV40 | T0030

Immortalized Human Synovial Membrane Fibroblasts -SV40 | T0030

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Synovial Membrane

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Multipolar

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

7,000 - 10,000 cells per cm10²

Markers:

N/A

Donor Age:

40

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow III medium available at abm. to a final concentration of 10%, 1% L-glutamine to a final concentration of 2mM, and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C