Immortalized Mouse TLR4^-/- Hepatic Stellate Cells | T0689

Immortalized Mouse TLR4^-/- Hepatic Stellate Cells | T0689

-/- mouse via enzymatic digestion and Percoll density gradient centrifugation. The primary cells were immortalized via transfection of the pCMV Simian Virus 40 Large T antigen and hygromycin B selection. The Immortalized Mouse TLR4^-/- Hepatic Stellate Cells are useful in studies focusing on liver fibrosis, especially in the role of the pro-inflammatory TLR4 activation in scar tissue formation. TLR4 lentivirus-mediated knock-in experiments have been performed on Plenti4/TO/V5-DEST Gateway^TM vectors (Invitrogen) and lipofectamine^TM 2000 (Thermo Fisher Scientific). The cells can be used in studies of cellular survival regulation, as TLR4 activation has been linked to apoptosis-resistance.

Immortalization Method:

Transfection of CMV Simian Virus 40 Large T antigen

BioSafety Level:

II

Organism:

TLR4^-/- mouse

Species:

Mouse

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Genotype analysis; 2)NF-κB Activation Assay; 3) Cytokine ELISA Assays; 4) ³[H]-thymidine Cell Proliferation Assay

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C