Immortalized Porcine Alveolar Macrophage Cells (iPAM303) | T0754

Immortalized Porcine Alveolar Macrophage Cells (iPAM303) | T0754

Immortalization Method:

Immortalized with pBABE-SV40LT-Puro and pBABE-hTERT-Puro using PolyMag kit

BioSafety Level:

II

Organism:

Pig (Porcine)

Species:

Pig (Porcine)

Source Organ:

Lung

Organ Type:

Lung

Growth Properties:

Adherent

Morphology:

Fibroblast-like

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

Puromycin resistance

Donor Age:

10 weeks old

Donor Gender:

Not disclosed

Donor Ethnicity:

Not disclosed

Propagation:

The base medium for this cell line is Prigrow II medium available at abm. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) PCR; 2) Immunohistochemistry; 3) Analysis of peptide binding to SLA class II molecules

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C