Immortalized Rat Embryonic Fibroblasts (18IM) | T0354

Immortalized Rat Embryonic Fibroblasts (18IM) | T0354

FUT4 and vimentin, and an up-regulation of stem cell markers, Sox2 and Oct3/4. This cell line may be useful as a model for the role of S18-2 in other cell types and further studies in the binding partners of S18-2 in differentiated tissues.

Immortalization Method:

Transfection of plasmid encoded with GFP-fused S18–2 (GFP-S18–2) carrying a neomycin resistance gene

BioSafety Level:

II

Organism:

Rat

Species:

Rat

Source Organ:

Embryo

Organ Type:

Reproductive

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

46-56 hours

Seeding Density:

20,000 – 100,000 cells/cm10²; Split Ratio: 1:2 or 1:3

Markers:

SSEA-1, Sox2 and Oct3/4

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow V medium available at abm (TM015). To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 5%, 0.5mg/ml Geneticin (Antibiotic G418) and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Western blot analysis for presence of S18-2 in cells; 2) Immunostaining for expression of markers ; 3) Reverse Transcription PCR for determination of presence of markers

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C