Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T | T0356

Immortalized Rat Embryonic Striatal (M213-2O) Cells - tsSV40T | T0356

Immortalization Method:

Immortalized via infection with retrovirus carrying the A58 temperature-sensitive allele of SV40 large T antigen

BioSafety Level:

II

Organism:

Sprague-Dawley rat embryo

Species:

Rat

Source Organ:

Straitum

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

N/A

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV medium available from abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 33.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Immunostaining for SV40 T large antigen and markers; 2) Test growth in permissive and non-permissive temperatures

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C