Immortalized Rat Enteroglial Cells (EGC) | T0245

Immortalized Rat Enteroglial Cells (EGC) | T0245

c-fos mRNA but can be up-regulated with stimuli from pro-inflammatory cytokines (IL-1β and TNF-α).

Immortalization Method:

Serial passaging and infection by incubating with the supernatant of virus-producing GPE-egfr-neu cell line

BioSafety Level:

II

Organism:

Sprague-Dawley Rat

Species:

Rat

Source Organ:

Jejunum

Organ Type:

Brain

Growth Properties:

Adherent

Morphology:

Stellate shaped

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.

Markers:

N/A

Donor Age:

N/A

Donor Gender:

Male

Donor Ethnicity:

N/A

Propagation:

Propagation: Grow cells in T25 ECM-coated flasks (G299) with the following conditions. The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 5% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.

Quality Control:

Characterized by immunohistochemistry and dot-blot analysis.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C