Inducible NOS, Mouse Recombinant | P1667

Inducible nitric oxide synthase (iNOS), also known as inflammatory nitric oxide synthase, is a calcium independent isoenzyme involved in synthesis of nitric oxide (NO). It is a soluble enzyme encoded by the gene mapped to mouse chromosome 11. iNOS is active in dimeric form and its activity is induced by cytokines and various other stimuli, including lipopolysaccharide. iNOS is expressed in various inflammatory conditions and enhances the synthesis of proinflammatory mediators such as IL-6 and IL-8.

P1667 | Inducible NOS, Mouse Recombinant DataSheet

Biomolecule/Target :

Synonyms: Inducible Nitric Oxide Synthase; iNOS; NOS2

Alternates names: Inducible Nitric Oxide Synthase; iNOS; NOS2

Taglines: Involved in various inflammatory conditions

NCBI Gene ID #: 18126

NCBI Gene Symbol:

Gene Source: Human

Accession #: P29477

Recombinant: True

Source: E.coli

Purity by SDS-PAGE: > 80% by SDS - PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): ≥ 100 mU/mg. Measured by its ability to convert L-arginine to nitric oxide and L-citrulline by using ’s Nitric Oxide Synthase (NOS) Activity Assay Kit (Cat. No. K205).

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of NOS is defined as the amount of enzyme that generates 1 umol of nitric oxide per min at 37 °C under the assay conditions.

Molecular Weight: 131.6 kDa with N-terminal His tag

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: aa 1-1144 (P29477)

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans