INPP4B Antibody | 8339

INPP4B Antibody | 8339 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: INPP4B antibody was raised against a 17 amino acid peptide near the amino terminus of human INPP4B.
The immunogen is located within the last 50 amino acids of INPP4B.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, ICC

Application: INPP4B antibody can be used for detection of INPP4B by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: INPP4B antibody is human, mouse and rat reactive. At least two isoforms of INPP4B are known to exist; this antibody only detects the longer isoform. INPP4B antibody is predicted to not cross-react with INPP4A.

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: Cat. No. 17-201 - 3T3/BALB Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 102 kDa
Observed: 100 kDa

Validation: N/A

Isoform: N/A

Purification: INPP4B antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: INPP4B antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: INPP4B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Inositol polyphosphate-4-phosphatase type II 105kDa

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Inositol polyphosphate 4-phosphatase type II (INPP4B) is one of the enzymes involved in phosphatidylinositol signaling pathways. It removes the phosphate group at position 4 of the inositol ring from inositol 3, 4-bisphosphate (1) . INPP4B can function as a tumor suppressor by inhibiting PI3K signaling. In human epithelial cells, knockdown of INPP4B expression resulted in enhanced Akt activation, anchorage-independent growth, and enhanced overall motility (2) . It is thought INPP4B may also have oncogenic functions as INPP4B-dependent PI3K signaling is mediated by SGK3 in breast cancer cells and expression of INPP4B leads to enhanced SGK3 activation and suppression of AKT phosphorylation (3) .