INS, 25-110 aa, His-tag, Human Recombinant | P1302

INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

P1302 | INS 25-110 aa His-tag Human Recombinant DataSheet

Biomolecule/Target: INS

Synonyms: Insulin preproprotein , IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10

Alternates names: HBGF-7, FGF7, FGF-7, KGF

Taglines: Essential hormone for maintaining metabolic homeostasis

NCBI Gene ID #: 2252

NCBI Gene Symbol: FGF7

Gene Source: Human

Accession #: P21781

Recombinant: Yes

Source: Human cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: ED is typically 1.5 to 7.5 ng/mL. The specific activity was determined by the Dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Results: 1.5 to 7.5 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17 and 30 kDa, monomer, glycosylated

Concentration: 0.5 mg/ml (determined by Absorbance at 280nm)

Appearance: Liquid

Physical form description: In Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

Amino acid sequence: N/A