Integrin alpha 4 Antibody | 4837

Integrin alpha 4 Antibody | 4837 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Integrin alpha 4 antibody was raised against a 15 amino acid synthetic peptide from near the center of human Integrin alpha 4.
The immunogen is located within amino acids 220 - 270 of Integrin alpha 4.

Research Area: Infectious Disease

Tested Application: E, WB, IF

Application: Integrin alpha 4 antibody can be used for the detection of Integrin alpha 4 by Western blot at 1 μg/mL. Despite its predicted molecular weight, Integrin alpha4 will often run at higher molecular weight in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 2 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms are known to exist. The immunogen to this antibody is within the Integrin alpha4 recombinant protein fragment and thus will recognize it.

Positive Control 1: Cat. No. 1466 - Rat Spleen Tissue Lysate

Positive Control 2: Cat. No. 17-005 - Jurkat Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Integrin alpha 4 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Integrin alpha 4 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Integrin alpha 4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Integrin alpha 4 Antibody: The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains. Alpha4 chain associates with either beta1 or beta7 chain. It has been demonstrated that the putative ligand-binding sites of both integrin alpha4beta1 and alpha4beta7 is located on the alpha4 chain. These ligands included Madcam, VCAM, and fibronectin. Madcam is known as the principal ligand for integrin alpha4beta7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells.