Ipaf Antibody | 3107

Ipaf Antibody | 3107 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (79%) , Mouse: (71%)

Immunogen: Ipaf antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human Ipaf.
The immunogen is located within the last 50 amino acids of Ipaf.

Research Area: Apoptosis,

Tested Application: E, WB, ICC, IF

Application: Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 μg/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate

Positive Control 2: Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 3: Cat. No. 17-008 - THP-1 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 110 kDa

Validation: N/A

Isoform: N/A

Purification: Ipaf Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Ipaf Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Ipaf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Ipaf Antibody: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1, CLAN, UNQ6189/PRO20215, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Clan protein

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Ipaf Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NFκ signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.