IPG-1 AM | B3140

IPG-1 is a yellow-green fluorescent, intracellular potassium (K⁺) indicator for measuring changes at most intracellular K⁺ concentrations. It is a small, synthetic fluorochrome which incorporates a K⁺-binding moiety. When K⁺ binds, the quenching is relieved and the fluorescence dramatically increases. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K⁺ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Cat. Nos. B3142, B3143; Kd = 18 mM) or IPG-4 (Cat. Nos. B3144, B3145; Kd = 7 mM). IPG-1 responds to modest changes in intracellular K⁺ concentrations resulting from activation of plasma membrane K⁺ channels. IPG-1 AM is the membrane permeable form of the potasssium indicator (Cat. No. B3141). Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. IPG-1 is compatible with a wide variety of detectors including fluorescent microscopes, plate readers, flow cytometers, and fluorescent indicator-doped solid-state sensors.

IPG-1 is a yellow-green fluorescent, intracellular potassium (K⁺) indicator for measuring changes at most intracellular K⁺ concentrations. It is a small, synthetic fluorochrome which incorporates a K⁺-binding moiety. When K⁺ binds, the quenching is relieved and the fluorescence dramatically increases. IPG-1 has Ex/Em: 525 nm/545 nm and a high-sensitivity to detect small changes in K⁺ concentration. IPG-1 has a lower affinity (Kd = 50 mM) than IPG-2 (Cat. Nos. B3142, B3143; Kd = 18 mM) or IPG-4 (Cat. Nos. B3144, B3145; Kd = 7 mM). IPG-1 responds to modest changes in intracellular K⁺ concentrations resulting from activation of plasma membrane K⁺ channels. IPG-1 AM is the membrane permeable form of the potasssium indicator (Cat. No. B3141). Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. IPG-1 is compatible with a wide variety of detectors including fluorescent microscopes, plate readers, flow cytometers, and fluorescent indicator-doped solid-state sensors.

B3140 | IPG-1 AM DataSheet

Alternate Name/Synonyms: Potassium (K⁺) indicator; IPG-1 membrane permeable; IPG-1 acetoxymethyl ester; Fluorescent Potassium indicator

Appearance: Red-orange powder

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Structure Available?: TRUE

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Salt Form: FALSE

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Molecular Weight: 1140

Cell-Permeable?: TRUE

Purity: ≥ 90%

Solubilities: ~2 mg/ml in DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A membrane permeable fluorescent potassium indicator

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