IRAK-2 Antibody | 2123

IRAK-2 Antibody | 2123 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (77%)

Immunogen: IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.
The immunogen is located within amino acids 530 - 580 of IRAK-2.

Research Area: Signal Transduction

Tested Application: E, WB, ICC, IF

Application: IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 μg/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Anti-IRAK2 has no cross response to IRAK.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 1204 - K562 Cell Lysate

Positive Control 3: Cat. No. 17-001 - HeLa Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 65 kDa

Validation: N/A

Isoform: N/A

Purification: IRAK-2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: IRAK-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IRAK-2 Antibody: IRAK-2, Interleukin-1 receptor-associated kinase-like 2, IRAK-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) . IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.