IRAK-M Antibody | 2355

IRAK-M Antibody | 2355 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: IRAK-M antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M.
The immunogen is located within the last 50 amino acids of IRAK-M.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P, IF

Application: IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Specificiy: IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.

Positive Control 1: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 2: Cat. No. 1465 - Rat Kidney Tissue Lysate

Positive Control 3: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 66 kDa
Observed: 70 kDa

Validation: N/A

Isoform: N/A

Purification: IRAK-M Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IRAK-M Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IRAK-M antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IRAK-M Antibody: ASRT5, IRAKM, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAK-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IRAK-M Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR) . IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.