IRF7 Antibody | 8991

IRF7 Antibody | 8991 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-IRF7 antibody (8991) was raised against a peptide corresponding to 17 amino acids near the center of human IRF7.
The immunogen is located within amino acids 200-230 of IRF7.

Research Area: Innate Immunity

Tested Application: E, WB, IHC-P

Application: WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples. Immunofluorescence in mouse samples. Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.

Specificiy: Several isoforms of IRF7 are known to exist.

Positive Control 1: Cat. No. 1203 - A549 Cell Lysate

Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation:

KO Validation (Figure 1) : Anti-IRF7 antibodies (8991) specificity was further verified by IRF7 specific knockout. IRF7 was disrupted in IRF7 knockout A549 cells as compared to that in control wild type cells.

Isoform: Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD) , isoform B (474aa, 52kD) , isoform C (164aa, 18kD) and isoform D (516aa, 56kD) . Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD) . 8991 can detect human isoforms and mouse, and possibly can detect rat.

Purification: IRF7 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: IRF7 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: IRF7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: IRF7 Antibody: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: IRF7 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.