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IRS-1 (Phospho-Ser794) polyclonal Antibody | AP0086
- SKU:
- BW-AP0086
- Availability:
- Usually ships in 5 working days
Description
IRS-1 (Phospho-Ser794) polyclonal Antibody | AP0086 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Application: WB
Application Range: WB: 1:500~1:1000
Background: Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IRS-1 (Phospho-Ser794) polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser794.
Molecular Weight: ~ 180 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Insulin receptor substrate 1; IRS-1; IRS1
Immunogen: Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 794.
Conjugate: Unconjugated
Modification: Phosphorylation
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,MTOR signaling,