IRS-1 (Phospho-Ser794)  polyclonal Antibody | AP0086

IRS-1 (Phospho-Ser794)  polyclonal Antibody | AP0086 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB

Application Range: WB: 1:500~1:1000

Background: Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IRS-1 (Phospho-Ser794) polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser794.

Molecular Weight: ~ 180 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Insulin receptor substrate 1; IRS-1; IRS1

Immunogen: Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 794.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,MTOR signaling,