Isocitrate Dehydrogenase 1 R132H mutant, Recombinant Human | P1070

Mutations of the cytosolic IDH 1 are a common feature in primary human brain cancers. Arginine 132 (R132) of IDH is highly conserved among different isoforms of IDH and is most commonly mutated to Histidine. Mutation of IDH1 R132H leads to accumulation of R(-)-2-hydroxyglutarate (2HG), which correlates with an increased risk for malignant brain tumors.

P1070 | Isocitrate Dehydrogenase 1 R132H mutant Recombinant Human DataSheet

Biomolecule/Target: N/A

Synonyms: Isocitrate dehydrogenase [NADP] cytoplasmic, R132H, IDH 1 R132H

Alternates names: CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Taglines: Catalyzes the rate-limiting step in Citric acid cycle

NCBI Gene ID #: 1437

NCBI Gene Symbol: CSF2

Gene Source: Human

Accession #: P04141

Recombinant: Yes

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: ED is typically 0.4 to 2 ng/mL. The specific activity was determined by the dose- Dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic Indicator cell line).

Results: 0.4 to 2 ng/ml

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme that will convert 1.0 µmole of NADPH to NADP+ per minute at pH 7.4 at 25 C.

Molecular Weight: 15 to 36 kDa, monomer, glycosylated

Concentration: N/A

Appearance: Lyophilized

Physical form description: N/A

Reconstitution Instructions: Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

Amino acid sequence: N/A