Description
IVD (Isovaleryl Coenzyme A dehydrogenase) is a mitochondrial matrix enzyme that belongs to the acyl-CoA dehydrogenase family. IVD is a homotetrameric flavoenzyme which catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA. Defects of the IVD gene lead to ineffective isoforms that are the underlying cause of isovaleric acidemia. Recombinant human IVD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
P1577 | IVD, Human Recombinant DataSheet
Biomolecule/Target :
Synonyms: Isovaleryl-CoA dehydrogenase mitochondrial, ACAD2
Alternates names: Isovaleryl-CoA dehydrogenase mitochondrial, ACAD2
Taglines: Flavoenzyme which catalyzes the conversion of isovaleryl-CoA to 3-methylcrotonyl-CoA
NCBI Gene ID #: 3712
NCBI Gene Symbol: IVD
Gene Source: Human
Accession #: P26440
Recombinant: TRUE
Source: E. coli
Purity by SDS-PAGE: >90%
Assay: SDS - PAGE
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method):
Biological activity:
Results:
Binding Capacity:
Unit Definition:
Molecular Weight: 45.3 kDa (415aa) (N-terminal His- Tag)
Concentration:
Appearance: Liquid
Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Reconstitution Instructions:
Amino acid sequence: aa 33-426
Handling: Centrifuge the vial prior to opening.
Usage: For Research Use Only! Not to be used in humans
Additional Information
Storage Temperature: |
-20°C |
Shipping: |
Gel Pack |
Shelf Life: |
12 months |