IWP-4 | 2650

IWP-4 is a potent inhibitor of Wnt production that blocks Wnt pathway activity in vitro (IC₅₀ = 25 nM). IWP-4 inactivates Porcupine (porcn), a membrane-bound O-acyltransferase responsible for palmitoylating Wnt proteins, which is essential for their signaling ability and secretion. At 5 μM, IWP-4 has been shown to block Wnt-dependent phosphorylation of the low-density lipoprotein receptor-related protein 6 receptor and the scaffold protein Dishevelled, preventing the accumulation of β-catenin. IWP-4 has been used to induce cardiomyocyte differentiation from human pluripotent stem cells.

IWP-4 is a potent inhibitor of Wnt production that blocks Wnt pathway activity in vitro (IC₅₀ = 25 nM). IWP-4 inactivates Porcupine (porcn), a membrane-bound O-acyltransferase responsible for palmitoylating Wnt proteins, which is essential for their signaling ability and secretion. At 5 μM, IWP-4 has been shown to block Wnt-dependent phosphorylation of the low-density lipoprotein receptor-related protein 6 receptor and the scaffold protein Dishevelled, preventing the accumulation of β-catenin.

2650 | IWP-4 DataSheet

Alternate Name/Synonyms: N-​(6-​methyl-​2-​benzothiazolyl)-​2-​[[3,​4,​6,​7-​tetrahydro-​3-​(2-​methoxyphenyl)-​4-​oxothieno[3,​2-​d]pyrimidin-​2-​yl]thio]-​acetamide

Appearance: Crystaline solid

Formulation: N/A

CAS Number: 686772-17-8

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₃H₂₀N₄O₃S₃

Molecular Weight: 496.62

Cell-Permeable?: Yes

Purity: ≥95% by HPLC

Solubilities: DMSO (~2 mg/ml)

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A Wnt pathway antagonist

MDL Number: N/A

PubChem CID: N/A

SMILES: O=C(NC1=NC2=CC=C(C)C=C2S1)CSC3=NC(CCS4)=C4C(N3C5=CC=CC=C5OC)=O

InChi: InChI=1S/C23H20N4O3S3/c1-13-7-8-14-18(11-13)33-22(24-14)26-19(28)12-32-23-25-15-9-10-31-20(15)21(29)27(23)16-5-3-4-6-17(16)30-2/h3-8,11H,9-10,12H2,1-2H3,(H,24,26,28)

InChi Key: RHUJMHOIQBDFQR-UHFFFAOYSA-N