izTRAIL, Soluble Recombinant Protein | 90-257

izTRAIL, Soluble Recombinant Protein | 90-257 | Gentaur UK, US & Europe Distribution

Tested Application: N/A

Application: This recombinant proteins is for research use only.

Tpredicted Molecular Weight: N/A

Physical State: Lyophilized

Buffer: Contains 20mM TRIS-Cl, 0.5M arginine-HCl, 100mM NaCl, 0.02% Tween 20. Reconstitute with 100 μl sterile water.

Storage Condition: Stable for at least 6 months after receipt when stored at -20˚C.

Alternate Name: Apo-2L, TNFSF10, CD253

Background: N/A

Shipping: N/A

Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

Source: E. coli

Species: Human

By Source: E. Coli

By Species: Human

Fusion Tag: isoleucine zipper motif Tag

Sequence: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to an isoleucine zipper motif.

Bological Activity: Induces apoptosis in vitro in different human cancer cell lines with an EC50 of 5-200ng/ml, depending on the individual cell line used. Recombinant izTRAIL does not kill 4-day cultures of primary human hepatocytes (PHH) at concentrations of at least up to 1ug/ml (Ganten 2006). Good bioavailability in vivo, shows no toxic effects in mice at doses of at least up to 500ug per day (Wissink 2006).

Purity: >95% (SDS-PAGE).

Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).