Jacalin | 6560

Jacalin is isolated from Jackfruit seeds and purified by affinity chromatography. It is a glycoprotein with two non-covalently bound subunits (14.6 and 17.1 kDa) and belongs to the family of galactose-binding lectins. Jacalin is preferably used in applications to isolate IgA from human serum, isolating human plasma glycoproteins and for applications in histochemistry. ’s Jacalin is supplied as a white to light-yellow lyophilized powder without additives. The purity of the lectin is determined by SDS-PAGE, generating two homogeneous bands at 14 kDa and 17 kDa. Gene Bank Accession Number Q38720.

6560 | Jacalin DataSheet

Biomolecule/Target: N/A

Synonyms: Jacalin

Alternates names: Macrophage Colony Stimulating Factor, CSF-1, Lanimostim, MCSF, MGC31930, M-CSF.

Taglines: Galactose-binding lectin to purify IgA

NCBI Gene ID #: 1435

NCBI Gene Symbol: CSF1

Gene Source: Human

Accession #: P09603

Recombinant: No

Source: Human 293 cell expressed

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 0.1 EU/mg

Activity (Specifications/test method): N/A

Biological activity: The EC50 is typically 0.5 to 4 ng/mL for this protein with specific activity determined by the dose dependent stimulation of the proliferation of murine M-NFS-60 cells (Mouse Myeloid Leukemia indicator cell line)

Results: 0.5 to 4 ng/ml

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: This protein has 223 amino acids (Glu 33 - Arg 255) with a 6x-His tag at C-terminus, and a calculated MW of 26 kDa. The protein migrates to 35-45 kDa under reducing and non-reducing conditions (SDS-PAGE) due to glycosylation

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized without additives

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile PBS (pH 7.4). Do not vortex.

Amino acid sequence: N/A