JAG1 Antibody | 8059

JAG1 Antibody | 8059 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: JAG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human JAG1.
The immunogen is located within the last 50 amino acids of JAG1.

Research Area: Cancer, Cell Cycle, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: JAG1 antibody can be used for detection of JAG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 134 kDa
Observed: 130 kDa

Validation: N/A

Isoform: N/A

Purification: JAG1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: JAG1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: JAG1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Jagged 1, AGS, AHD, AWS, CD339, HJ1, JAGL1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The LIN-12/Notch family of transmembrane receptors plays a central role in development by regulating cell fate decisions (1) . Ligands for Notch include JAG1, JAG2 and Delta. JAG1 is one of the DSL (Delta, Serrate, Lag2) transmembrane proteins that can activate Notch. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis (1, 2) . JAG1 is widely expressed in adult and fetal tissues and the mutation of the JAG1 gene is associated with Familial Tetrology of Fallot (3) . JAG1 expression is associated with prostate cancer metastasis and is up-regulated in cervical squamous cell carcinoma (4, 5) .